The effect of inclination on the steady natural convection local heat transfer characteristics in an air-filled differentially heated enclosure with cross-sectional aspect ratio one is studied experimentally and numerically. Measurements of local and mean Nusselt numbers are obtained at various inclination angles, ranging between 0° (heated from above) and 180° (heated from below), for Rayleigh numbers between 104 and 106 and are compared with the numerical predictions. The heat flux at the hot and cold boundaries show strong dependence on the angle of inclination and the Rayleigh number. In addition, new results and details are made available concerning the local heat transfer distributions as a function of the inclination angle and Rayleigh number. Flow patterns, stream functions and isotherms at different inclination angles are shown in order to give new insight and greater understanding of the flow and heat transfer behavior.